We are delighted to see our first collaboration with members of the Biology Department and IMBB-FORTH bear fruit.
Our work in collaboration with the lab of Despoina Alexandraki from the Dept of Biology at the University of Crete and IMBB-FORTH, entitled "Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae" was published last week in Nucleic Acids Research.
The work forms the basis of the PhD thesis of Christos Andreadis, a former student of the Dept of Biology, currently at UCL, and deals with how the DNA damage checkpoint protein Rad9 exhibits a more versatile role than simply signalling for DNA damage. Through a series of elegant experiments including co-localization of Rad9 with the transcription factor Aft1, expression profiling upon Rad9 deletion and genome-wide localization of Rad9, Christos showed that Rad9 also plays a surveillance role in the yeast genome, being preferentially stationed at "high-risk" regions, such as highly expressed genes, sites of origin of replication and recombination hotspots.
CG2's contribution in this work was primarily related to the analysis of primary data, the extraction of genome-wide profiles of protein binding from ChIP-on-chip experiments and the statistical inference of genomic co-localizations.
You can access the paper freely here, while you may read more about it in the Publications page.
Our work in collaboration with the lab of Despoina Alexandraki from the Dept of Biology at the University of Crete and IMBB-FORTH, entitled "Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae" was published last week in Nucleic Acids Research.
The work forms the basis of the PhD thesis of Christos Andreadis, a former student of the Dept of Biology, currently at UCL, and deals with how the DNA damage checkpoint protein Rad9 exhibits a more versatile role than simply signalling for DNA damage. Through a series of elegant experiments including co-localization of Rad9 with the transcription factor Aft1, expression profiling upon Rad9 deletion and genome-wide localization of Rad9, Christos showed that Rad9 also plays a surveillance role in the yeast genome, being preferentially stationed at "high-risk" regions, such as highly expressed genes, sites of origin of replication and recombination hotspots.
CG2's contribution in this work was primarily related to the analysis of primary data, the extraction of genome-wide profiles of protein binding from ChIP-on-chip experiments and the statistical inference of genomic co-localizations.
You can access the paper freely here, while you may read more about it in the Publications page.
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